cyanine 7 5 cy7 5 labeled ha  (Lumiprobe)

Journal: Nature Communications

Article Title: Sprayable anti-adhesive hydrogel for peritoneal macrophage scavenging in post-surgical applications

doi: 10.1038/s41467-024-52753-0

Figure Lengend Snippet: a Schematic illustration of the in vivo biocompatibility imaging of sHAChiF with various formulations by luminol assay. b Representative IVIS images of bright field (left) and bioluminescence (right) of luminol activity for implanted sHAChiF in C57BL/6 mice (Scale bar = 10 mm). c Quantification of bioluminescence efficiencies of luminol activities for implanted sHAChiF in the mice. d Representative IVIS images of fluorescence by Cy5.5-conjugated Chi (Chi Cy5.5 ; cyan; pseudocolor), and Cy7.5-conjugated sHA (sHA Cy7.5 ; magenta; pseudocolor) in sHAChiF at various time points (Scale bar = 10 mm). e In vivo retention profiles of Chi Cy5.5 and sHA Cy7.5 in sHAChiF over time through calculation of fluorescence intensity, normalized by fluorescence at 0 d. f Quantification of normalized sHAChiF in vivo retention at 7 d and 14 d. Data are shown as mean ± SD (n = 3) independent samples in b , c ; n = 4 independent samples in d – f . One-way ANOVA with Tukey post-hoc test was used for statistical analysis of c , f . Source data are provided as a Source Data file. Image was created using PowerPoint.

Article Snippet: Cyanine5.5 (Cy5.5) NHS ester (Catalog # 27020) and Cyanine7 (Cy7) amine (Catalog # 260C0) were purchased from Lumiprobe (USA).

Techniques: In Vivo, Imaging, Activity Assay, Fluorescence

Journal: Nature Communications

Article Title: Sprayable anti-adhesive hydrogel for peritoneal macrophage scavenging in post-surgical applications

doi: 10.1038/s41467-024-52753-0

Figure Lengend Snippet: a Representative images of the aggregation behavior of rat peritoneal macrophages following treatment with various polysaccharides (Scale bar = 50 μm). b Quantification of aggregated rat peritoneal macrophages treated with various polysaccharides. c Schematic illustration of the mouse hepatic thermal injury model. d Representative images of mouse organs in the mouse hepatic thermal injury model after treatment with sHAChiF, showing F4/80 (yellow; pseudocolor), Chi (cyan; pseudocolor), and sHA (magenta; pseudocolor) (Scale bar = 10 mm). e Quantification of F4/80 expression on the wound by measuring fluorescence with APC/Fire TM anti-F4/80 antibody, normalized by fluorescence levels in the normal liver. f Quantification fluorescence of sHA from sHA Cy7.5 on the wound (left) and Chi from Chi Cy5.5 on the wound (right), normalized by fluorescence from normal liver. g Schematic illustration of analysis for sHA uptake behavior of peritoneal cells using flow cytometry. Peritoneal cells gated based on sHA + population ( h ), grouping of sHA + LPM and sHA + SPM by F4/80 and CD11b expression levels ( i ), the ratio of peritoneal cell populations distinguishing between sHA - and sHA + populations ( j ). Data are shown as mean ± SD ( n = 3) independent samples in a , b ; n = 4 independent samples in d – f ; n = 4 independent samples in h – j . Two-tailed unpaired t-test was used for statistical analysis of f . One-way ANOVA with Tukey post-hoc test was used for statistical analysis of b , e . Source data are provided as a Source Data file. Image was created using PowerPoint.

Article Snippet: Cyanine5.5 (Cy5.5) NHS ester (Catalog # 27020) and Cyanine7 (Cy7) amine (Catalog # 260C0) were purchased from Lumiprobe (USA).

Techniques: Expressing, Fluorescence, Flow Cytometry, Two Tailed Test

Journal: Nature Communications

Article Title: Sprayable anti-adhesive hydrogel for peritoneal macrophage scavenging in post-surgical applications

doi: 10.1038/s41467-024-52753-0

Figure Lengend Snippet: a Schematic illustrations of modeling rat ischemic button model with sHAChiF (top) and representative images of sHAChiF treated on the ischemic buttons (Scale bar = 10 mm). b Representative images of rat ischemic button model on d 0 and d 7 after surgery (Scale bar = 10 mm). c Representative Masson’s trichrome staining images of adhesive tissues on d 7 after surgery (Scale bar = 1 mm). d Specification of scoring severity of peritoneal adhesion. e Adhesion formation rates with adhesion scores of sHAChiF per rat. f Quantification of the adhesion scores of dual-sprayable hydrogels. g Representative IVIS images of fluorescence by Chi Cy5.5 (cyan; pseudocolor) and sHA Cy7.5 (magenta; pseudocolor) in dual-sprayable hydrogels at various time points (Scale bar = 50 mm). h In vivo retention profiles of Chi Cy5.5 and sHA Cy7.5 in dual-sprayable hydrogels over time through calculation of fluorescence intensity, normalized by fluorescence at d 0. i Representative iNOS and Arg1 immunofluorescence staining images of adhesive tissues on d 7 after surgery (Scale bar = 50 μm). j Quantification of M1/M2 ratio of peritoneal macrophages in adhesive tissues on d 7 after surgery. Quantification of pro-inflammatory cytokines IL−1β ( k ), IL-6 ( l ), and TNF-α ( m ) from rat serum on d 4 after surgery. Data are shown as mean ± SD ( n = 5 independent samples in b – f ; n = 4 independent samples in g , h ; n = 5 independent samples in i , j ; n = 4 independent samples in k – m ). One-way ANOVA with Tukey post-hoc test was used for statistical analysis of e , f , j – m . Source data are provided as a Source Data file. Image was created using PowerPoint.

Article Snippet: Cyanine5.5 (Cy5.5) NHS ester (Catalog # 27020) and Cyanine7 (Cy7) amine (Catalog # 260C0) were purchased from Lumiprobe (USA).

Techniques: Staining, Adhesive, Fluorescence, In Vivo, Immunofluorescence